Detection of molecular colonies on the membrane by hybridization with fluorescent probes

摘要

1. A method for the detection and quantification of nucleic acids, comprising: (a) preparing a sample suspected of containing at least one kind of defined nucleic acid (target), (b) reproduction in a molecular target colonies by performing multiplication reaction in the immobilized medium, cell-free enzyme system comprising an exponential multiplication of nucleic acids, this target is able to propagate, (c) transferring the contents of molecular colonies on the membrane, (d) immobilizing suffered nnyh nucleic acids on the membrane, (d) detection of nucleic acids on the membrane by hybridization with a probe complementary to the target sequence, characterized in that a fluorescently-labeled probe and post hybridisation obtained two-dimensional fluorescence image of the membrane in the mode that provides registration zonda.2 fluorescence. A method according to claim 1, characterized in that a primer-dependent enzyme system exponential multiplication of nucleic acids comprising a target-specific praymery.3. A method according to claim 2, characterized in that as said primer-dependent systems use the polymerase chain reaction system and to carry out reproduction is carried out cyclical changes immobilized fluid temperature, this flow providing reaktsii.4. A method according to claim 3, characterized in that a target used DNK.5. A method according to claim 3, characterized in that is used as a target RNA, which is converted to DNA by reverse transkriptsii.6. A method according to claim 2, characterized in that as said primer of