In vitro automatic screening procedure for the determination of the developmental neurotoxicity of noxae on normal human neural brain cells, comprises culturing neurospheres under removal of growth factors and under addition of noxae

摘要

The In vitro automatic screening procedure for the determination of the developmental neurotoxicity (DNT) of noxae on normal human neural brain cells, comprises culturing neurospheres (1) under removal of growth factors and under addition of the concentrated noxae and immobilizing on coated slides, and microscopically determining the distance and/or the number of cells, which grow from the neurospheres in a time and which migrate radially from the spheres edge, from the sphere core and comparing with the distance of the migrating cells and/or their count. The In vitro automatic screening procedure for the determination of the developmental neurotoxicity (DNT) of noxae on normal human neural brain cells, comprises culturing neurospheres (1) under removal of growth factors and under addition of the concentrated noxae and immobilizing on coated slides, and microscopically determining the distance and/or the number of cells, which grow from the neurospheres in a time and which migrate radially from the spheres edge, from the sphere core and comparing with the distance of the migrating cells and/or their count, achieved under identical culture conditions in noxae-free cultures. The brain cells form functional neural networks during their natural development in the brain. The distance of the fully grown and toxically unaffected cells radially from the cell boundary is 0.3-0.4 mm, after a culturing time of 24 hours.