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Being the resin for chromatin immunity sinkage which is used in the chromatin immunity sedimentation method where
Being the resin for chromatin immunity sinkage which is used in the chromatin immunity sedimentation method where
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机译:作为染色质免疫沉淀的树脂,用于染色质免疫沉淀法中
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摘要
PROBLEM TO BE SOLVED: To provide a new resin for chromatin immunoprecipitation and a chromatin immunoprecipitation method using the same, for improving complexity in a DNA refinement process which is the process of a chromatin immunoprecipitation method, drastically shortening time necessary for an experiment, suppressing the consumption of a reagent, and providing satisfactory detection sensitivity.;SOLUTION: A resin, comprising a carrier coated with a protein and nucleic acid, is used to form a complex of resin + protein Y (such as Protein A/G) + anti-protein X antibody + protein X + DNA fragment. PCR amplification is performed without separating/collecting DNA fragments from the complex.;COPYRIGHT: (C)2005,JPO&NCIPI
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