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METHOD OF DESIGNING PROBES FOR DETECTING TARGET SEQUENCE AND METHOD OF DETECTING TARGET SEQUENCE USING THE PROBES
METHOD OF DESIGNING PROBES FOR DETECTING TARGET SEQUENCE AND METHOD OF DETECTING TARGET SEQUENCE USING THE PROBES
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机译:设计用于检测目标序列的问题的方法以及使用该问题检测目标序列的方法
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摘要
A method of designing probes for detecting a target sequence and a method of detecting target sequence using the probes are provided. The method of designing probes for detecting target sequence includes: selecting an anchoring location in the target sequence, wherein the anchoring location is a location in the target sequence at which a first non-target sequence in a plurality of non-target sequences is different from the target sequence, wherein the first non-target sequence has a sequence similarity to the target sequence that is identical to or higher than the sequence similarity of other non-target sequences in the plurality of non-target sequences; selecting a first probe designing region, wherein the first probe designing region is a fixed region in the target sequence comprising the anchoring location; selecting a probe selection location, wherein the probe selection location is a location in the target sequence at which a second non-target sequence in the plurality of non-target sequences is different from the target sequence; selecting a matched probe, wherein the matched probe comprises a sequence complementary to the target sequence, wherein the probe selecting location is at the center of the sequence of the matched probe; and selecting a mismatched probe, wherein the mismatched probe is longer than the matched probe, wherein the mismatched probe comprises a sequence complementary to the target sequence at all locations except at a mismatched location, wherein the probe selection location is at a first location in the sequence of the mismatched probe and the mismatched location is at a second location in the sequence of the mismatched probe.
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