Methods for purging ex vivo cells in autologous transplant, for the preparation of collected cells substantially free of malignant cells, collected cells, and a method for autologous transplant of cells.

摘要

Methods for purging ex vivo cells in autologous transplant, for the preparation of collected cells substantially free of malignant cells, collected cells, and a method for autologous transplant of cells.The present invention relates to a method for purging ex vivo cells in autologous transplant, in which the sample of collected cells is treated with a sufficient amount of a multimu00e9rica of soluble portion of FasL to kill the malignant cells without substantially affecting the VI Ability of cells to be transplanted.Autologous transplantation of stem cells (ASCT) after high-dose chemotherapy with or without radiotherapy became the standard therapy for most patients with large cell lymphomas, multiple myeloma, and Hodgkin's disease refractory / relapsed.This therapy is now also considered for selected patients lymphoma type low (chronic lymphocytic leukemia, follicular lymphoma, mantle cell lymphoma of) and for patients with acute myeloid leukemia (AML). The current treatments for purging of cells include chemotherapy and cocktail of antibodies.These treatments are often toxic to stem cells and not efficient in eliminating cancer cells. Thus, there is a medical need not answered for purging of cells in ASCT which this designs will be addressed.