Method for determination of acid metilmalu00c8nico based on extraction alquilativa associated with lu00ecquida chromatography coupled to mass spectrometry

摘要

Method for determination of acid metilmalu00c8nico based on extraction alquilativa associated with cromatograf1a lu00ecquida coupled to mass spectrometry.The present invention relates to a method for the determination of the presence of metilmalu00f3nico acid in biological samples, comprising the steps of extraction of the acid metilmalu00f3nico sample derivatisation of metilmalu00f3nico; acid; and use of the pre ionization mass spectrometry and The atmospheric pressure in the negative mode to determine the presence of the acid metilmalu00f3nico.An additional objective of this invention relates to diagnostic kits for determining the presence and quantification of metilmalu00f3nico acid based on the method mentioned before.