A METHOD OF PREPARING DIAKTSED AQUEOUS EXTRACT OF FENUGREEK SEEDS WHICH INDUCES HYPOGLYCEMIA, MEDIATED, IN PART, VIA STIMULATION OF INSULIN SIGNALING PATHWAY

摘要

A method of preparing dialysed aqueous extract of fenugreek seeds comprising washing the fenugreek seeds is distilled water, sterilizing the said seeds, subjecting the sterilized seeds to the step of grinding to form powder, suspending the said powder in phosphate buffered saline (PBS), subjecting the said suspension to the step of filtration to obtain the filtrate, treating the filtrate with activated charcoal to obtain clear supernatant, subjecting the supernatant to the step of lyophilization and the powder thus obtained was dissolved in phosphate buffered saline (PBS), dialyzing the aqueous extract of fenugreek seeds to obtain dialysed fenugreek seed extract (FSE) which was aliquoted and stored.