METHODS FOR THE IDENTIFICATION OF THE PIG WITH IMMUNITY AGAINST E. COLI F18 INTESTINE COLONIZATION OR INTESTINE DISORDERS RELATED TO E. COLI F18, ISOLATED DNA MOLECULES, A POLYPEPTIDE, A POLYNUCLEOTIDE, MOLECULAR DETERMINATION FOR THE DETECTION OF THE E. COLI F18 RECEPTORS, A KIT FOR THE DETECTION OF THE E. COLI F18 RECEPTORS AND USE OF MUTANT FORM OF THE ALPHA-1,2-FUCOSYL TRANSFERASE 1 GENE OF PIG

摘要

The present invention provides non-invasive methods and compositions to differentiate, with a high level of sensitivity and specificity, swine that are genetically susceptible to diseases associated with F18 E. coli infection, from resistant swine. DNA polymorphisms in the swine alpha (1,2) fucosyltransferase 1 (FUT1) gene were used to differentiate resistant from susceptible swine. The invention includes a polypeptide with amino acid substitutions, encoded by the nucleotide polymorphisms, a molecular diagnostic assay, and a kit for the differentiation, of E. coli F18-adhesion resistant, heterozygous (carrier) and homozygous susceptible pigs. The molecular test identifies susceptibility to oedema disease and postweaning diarrhea with high sensitivity and specificity, therefore, is useful to swine breeder in their effort to enhance for resistance. Information on the polymorphisms of the present invention provides insight into causation and treatment of E. coli associated intestinal disorders.