METHOD FOR REVEAL OF DNA OF VIRUS OF HORSE RHINO-PNEUMONIA OF 4 TYPE BY MEANS OF POLYMERASE CHAIN REACTION

摘要

Method for reveal of DNA of virus of horse rhino-pneumonia of 4 type by means of polymerase chain reaction includes reveal in samples under study of specific fragments of nucleic acid (DNA) of pathogenic organism by means of cluster version of polymerase chain reaction (PCR) fermentative reaction and two pairs of artificially synthesized oligonucleotides (primers). To carry out cluster version of PCR one performs two stages of reaction with use for each stage of given pair of primers, though with same programs of thermo-cycler, this increases sensitivity of indication system.