NUCLEID SEQUENCES OF MICOBACTERIA EXPORTED POLYPEPTIDES, VECTORS THAT UNDERSTAND AND APPLICATIONS FOR DIAGNOSIS AND TUBERCULOSIS.

摘要

Recombinant vector for screening, cloning and / or expression, characterized in that it replicates in mycobacteria and because it contains: 1) a functional replicon in mycobacteria; 2) a selection marker; 3) an indicator cassette comprising: a) a multiple cloning site (polylinker), b) possibly an active transcription terminator in the mycobacteria, upstream of the polylinker, c) a first nucleotide sequence encoding a marker for export and / or protein secretion, said nucleotide sequence being devoid of its initiation codon and its regulation sequences, and d) in the downstream direction, a second nucleotide sequence encoding a promoter activity marker contained in the cloned fragment, said nucleotide sequence being devoid of its initiation codon.