METHOD FOR TYPING AN INDIVIDUAL USING SHORT TANDEM REPEAT (STR) LOCI OF THE GENOMIC DNA

摘要

The invention relates to a novel STR typing strategy, which permits the simultaneous amplification and subsequent analysis of several (e.g. eleven) polymorphic systems with amplicon sizes of less than 270 bp. According to said method, after PCR amplification, the multiplex reaction is divided into two sets of STR multiplexes and is analysed separately. Said multiplex system was developed and tested specifically for use in forensic investigation, when only restricted quantities of DNA or badly decomposed DNA can be obtained, e.g. when isolated from telogen hair roots.