A NOVEL SOIL MICROORGANISM, A NOVEL BETA TRANSAMINASE SEPERATED FROM THE SOIL MICROORGANISM, A GENE ENCODING THE BETA TRANSAMINASE, AND A METHOD FOR PRODUCING ENANTIOMERICALLY PURE BETA AMINO ACIDS AND THEIR DERIVATIVES BY USING THE SAME

摘要

A novel microorganism primarily isolated from soil using a minimum culture medium including a beta amino acid as a single nitrogen source is provided to show the high optical selectivity and transaminase activity on a D-type beta amino acid. And a method for producing an L-type or a D-type beta amino acid is provided to be able to obtain the enantiomerically pure product using the same microorganism. A Mesorhizobium sp. LUK is deposited as a deposition no. KCCM-10752P. An L-type beta amino acid is produced at a temperature of 20-70 deg.C under pH of 5-10 by using the Mesorhizobium sp. LUK or a cell extract thereof as a bio-catalyst through optical resolution. A D-type beta amino acid is produced at a temperature of 20-70 deg.C under pH of 5-10 by using the Mesorhizobium sp. LUK or the cell extract thereof as a bio-catalyst through asymmetric synthesis. A transformed host cell is transformed by a recombinant DNA vector including a beta-transaminase gene encoding an amino acid sequence of SEQ ID : NO. 3 or a gene having more than 97% homology to the sequence of SEQ ID : NO. 3 and encoding a protein with the beta-transaminase activity. Further, an amino accepter for the optical resolution is selected from keto acid group.