TRANSGENIC PIG CLONAL SOMATIC CELL LINE EXPRESSING CD59 GENE TO INHIBIT HYPERACUTE REJECTION OCCURRED BY XENOTRANSPLANTATION AND A MANUFACTURING METHOD THEREOF

摘要

The clonal body cell system of a transgene pig of expression protection element CD59 genes is by inhibiting the cytotoxic destruction in xenotransplant to be arranged to minimize complement activation, so as to the inhibition for the hyperacute rejection that cell line can be used for occurring by xenotransplant. The clonal body cell system of one transgene pig, expression protection element CD59 genes (KCLRF-BP-00139) contain (new) the coding CD59 genes of an expression cassette pcDNA-CD59, the nucleotide sequence without SEQ ID: 1.A kind of method, the clonal body cell system of transgene pig for manufacturing expression protection element CD59 genes (KCLRF-BP-00139) include the following steps:: (1) separating a body cell: 2 and 3 from the pig of fetus by using the priming paint of SEQ ID NOs; (2) expression vector is prepared, contains a CD59 genes and converting expressing carrier enters body cell; (3) select and cultivate the body cell of conversion. In addition, CD59 genes are not SEQ IDs: 1.