A 3-hydroxybutyrate-3-hydroxypropionate-4-hydroxybutyrate-lactate tetrapolymer is provided to replace uses of the conventional synthetic plastics as a biodegradable polymer, and to be suitable for use in medical fields. A 3-hydroxybutyrate-3-hydroxypropionate-4-hydroxybutyrate-lactate tetrapolymer comprises lactate, 4-hydroxybutyrate, 3-hydroxypropionate, and 3-hydroxybutyrate as repeating units. A method for preparing the 3-hydroxybutyrate-3-hydroxypropionate-4-hydroxybutyrate-lactate tetrapolymer includes a step of culturing or cultivating a cell or plant having a gene of an enzyme, a phosphotransbutylase gene, a butyrate kinase gene, and a polyhydroxyalkanoate synthase gene, wherein the enzyme coverts lactate into lactyl-CoA and 3-hydroxyalkanoate into 3-hydroxyalkanoyl-CoA.
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