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53 53 TRANSFORMANT EXPRESSING A FUSION PROTEIN OF p53 TUMOR SUPPRESSOR PROTEIN AND A FLUORESCENCE PROTEIN AND METHOD FOR SCREENING SUBSTANCES RELATING TO THE ACTIVITY OF p53 TUMOR SUPPRESSOR PROTEIN USING SAME
53 53 TRANSFORMANT EXPRESSING A FUSION PROTEIN OF p53 TUMOR SUPPRESSOR PROTEIN AND A FLUORESCENCE PROTEIN AND METHOD FOR SCREENING SUBSTANCES RELATING TO THE ACTIVITY OF p53 TUMOR SUPPRESSOR PROTEIN USING SAME
The present invention relates to a method for using transformed cell lines expressing the fusion protein and the second fluorescent protein of the p53 tumor suppressor protein (tumor suppressor) and the first fluorescent protein, and this search for substances involved in the activity of the p53 tumor suppressor protein that, specifically, 5 'from the 3' direction with the amino acid valine 143 has been inactive mutant p53 tumor suppressor protein gene replacement with alanine of; A first fluorescent protein gene fused to the gene; IRES (internal ribosome entry site) ribosome binding sequences; And a recombinant expression vector which comprises a second fluorescent protein gene, and their expression is controlled by a single promoter; The expression vector is introduced into the stably transformed cell line; And using said transformed cell line to a method for searching a substance which increases or inhibits the activity of the p53 tumor suppressor protein. Recombinant expression vector, and transformed cell lines according to the present invention may be useful for screening an anticancer agent, adjuvant chemotherapy, and degenerative diseases related therapeutic agents as biosensors for searching a substance related to the activity of the p53 tumor suppressor protein.
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