Gene clone of recombinant GroEL originated from small brown planthopper and detection method of immunocapture RT-PCR for rice stripe virus using the GroEL protein

摘要

A recombinant protein derived from small brown planthopper is provided to be specifically coupled to thread-shaped rice stripe virus, thereby forecasting precise RSV outbreak, developing a transgenic plant having RSV resistance and being applied as various tools for detecting the RSV. A recombinant protein described in SEQ ID : NO. 2 is obtained by expressing a recombinant protein in a gene clone(deposition no. KACC-95060P) described in SEQ ID : NO. 1, which is constructed by inserting a GroEL gene isolated from small brown planthopper into an expression vector, and then purifying the protein using His-taq Ni-NTA agarose column and is characterized in that it is specifically coupled to thread-shaped RSV. A method for detecting an RSV gene comprises the steps of: (a) coating a reaction container which is PolySorp treated with the GroEL recombinant protein derived from the small brown planthopper or an antibody thereof with a coating solution; (b) treating the reaction solution with a detecting sample through coupling reaction with the with the RSV to immuno-capturing RSV particles; and (c) after isolating genes from the immuno-captured RSV particles, subjecting the isolated genes to RT-PCR to detect genes of the RSV.