Sequence-based DNA markers for evaluation of phylogenetic relationships and cultivar identification in Korean watermelon varieties

摘要

A DNA marker based on 43 sequences is provided to be usefully used for molecular genetic study of Korean watermelon species and also used for analysis of genetic polymorphism, similarity analysis and main morphological characteristic analysis of the watermelon. A marker for identifying species of watermelon comprises: at least one SSR(simple sequence repeat) marker selected from the group consisting of primer sets of SEQ ID : NOs. 1 and 2, SEQ ID : NOs. 3 and 4, SEQ ID : NOs. 5 and 6, SEQ ID : NOs. 7 and 8, SEQ ID : NOs. 9 and 10, SEQ ID : NOs. 11 and 12, SEQ ID : NOs. 13 and 14, SEQ ID : NOs. 15 and 16, SEQ ID : NOs. 17 and 18, SEQ ID : NOs. 19 and 20, SEQ ID : NOs. 21 and 22, SEQ ID : NOs. 23 and 24, SEQ ID : NOs. 25 and 26, SEQ ID : NOs. 27 and 28, and SEQ ID : NOs. 29 and 30; at least one SCAR(sequence characterized amplified region) marker selected from the group consisting of primer sets of SEQ ID : NOs. 31 and 32, SEQ ID : NOs. 33 and 34, SEQ ID : NOs. 35 and 36, SEQ ID : NOs. 37 and 38, SEQ ID : NOs. 39 and 40, SEQ ID : NOs. 41 and 42, SEQ ID : NOs. 43 and 44, SEQ ID : NOs. 45 and 46, SEQ ID : NOs. 47 and 48, SEQ ID : NOs. 49 and 50, SEQ ID : NOs. 51 and 52, SEQ ID : NOs. 53 and 54, SEQ ID : NOs. 55 and 56, and SEQ ID : NOs. 57 and 58; and at least one CAPS(cleaved amplified polymorphic sequence) marker selected from the group consisting of primer sets of SEQ ID : NOs. 59 and 60, SEQ ID : NOs. 61 and 62, SEQ ID : NOs. 63 and 64, SEQ ID : NOs. 65 and 66, SEQ ID : NOs. 67 and 68, SEQ ID : NOs. 69 and 70, SEQ ID : NOs. 71 and 72, SEQ ID : NOs. 73 and 74, SEQ ID : NOs. 75 and 76, SEQ ID : NOs. 77 and 78, SEQ ID : NOs. 79 and 80, SEQ ID : NOs. 81 and 82, SEQ ID : NOs. 83 and 84, and SEQ ID : NOs. 85 and 86. A method for identifying the watermelon species comprises the steps of: (a) isolating a genome DNA from a watermelon sample; (b) amplifying a target sequence using the isolated genome DNA as a template and at least one oligonucleotide primer set as a primer; and (c) detecting the amplified product. A kit for identifying the watermelon species comprises at least one of the oligonucleotide primer set and a reagent for amplification.