METHOD FOR QUANTITATIVE ANALYSIS OF PROLINE HYDROXYLATION-INDUCED INTERACTIONS BETWEEN HIF-1 AND VBC PROTEIN COMPLEX USING FLUORESCENCE POLARIZATION

摘要

The present invention relates to a method for analyzing the interaction of HIF-1 peptide and VBC protein using fluorescence polarization ratio, specifically 1) fluorescence in HIF-1 peptide comprising the fluorescent material is hydroxyproline (hydroxyproline) is attached an preparing a fluorescent probe attached to the material; 2) reacting the protein with VBC the fluorescent probe; 3) method for quantitative analysis in the form of HIF-1 protein conjugate-VBC by observing the changes in the fluorescence polarization ratio compared to fluorescence polarization of the fluorescent probe itself help to measure a degree of polarization of fluorescence of the reaction product; How to search for materials which would interfere the binding of HIF-1 peptide and VBC protein using the method; And a method for analyzing the activity of the (prolyl hydroxylase) prolyl silanol hydroxyl using the method. Analysis method of the present invention can be effectively applied to a high-speed search using the well plate can be simply analyze the interaction of the protein with VBC changes in HIF-1 peptide of the fluorescence polarization degree in accordance with the combined presence.