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extraction, amplification and sequential hybridization of fungal cells, dna and method for evidence of fungal cells in clinical material
extraction, amplification and sequential hybridization of fungal cells, dna and method for evidence of fungal cells in clinical material
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机译:真菌细胞的提取,扩增和顺序杂交,DNA以及临床材料中真菌细胞的证据方法
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摘要
Detecting fungal cells in clinical material, comprises extracting fungal DNA from whole blood and detecting the extracted fungal DNA, and/or identifying the fungal species from the extracted DNA. Also claimed is identifying a fungal species, comprising preparing a test soln. contg. at least segments of the DNA of the species to be identified, and detecting nucleotide sequences characteristic of the species in the soln.. Also claimed are the oligonucleotide probes (I)-(VI), TCTGGGTAGC CATTTATGGC GAACCAGGAC (I), TTCTGGCTAA CCCCAAGTCC TTGTGGCTTG (II), GTCTTTCCTT CTGGCTAGCC TCGGGCGAAC (III), GTTGGCCGGT CCATCTTTCT GATGCGTACT (IV), TTTCCTTCTG GCTAGCCTTT TTGGCGAACC (V), and CATGGCCTTC ACTGGCTGTG GGGGGAACCA (VI).
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