Device for separating constituents of liquid mixture by centrifugal liquid-liquid column chromatography to extract and purify the constituents, comprises downstream of the column for maintaining back pressure higher than determined value

摘要

The device for separating constituents of a liquid mixture by centrifugal liquid-liquid column chromatography to extract and purify the constituents, comprises a downstream of the column having a unit to maintain back pressure higher than a determined value, and an upstream entry of the column having a unit to distribute injected fluids, sample to be separated, and lighter and heavier fluids. The maintenance unit comprises elements such as a check valve, a conduit with determined size for creating an energy loss and a pressure regulator. The device for separating constituents of a liquid mixture by centrifugal liquid-liquid column chromatography to extract and purify the constituents, comprises a downstream of the column having a unit to maintain back pressure higher than a determined value, and an upstream entry of the column having a unit to distribute injected fluids, sample to be separated, and lighter and heavier fluids. The maintenance unit comprises elements such as a check valve, a conduit with determined size for creating an energy loss and a pressure regulator. A value of back pressure is higher than degassing pressure of the liquids circulating in the column. An automaton controls a functioning of an injection pressure of the distribution unit. An independent claim is included for a method for separating constituents of a liquid mixture by centrifugal liquid-liquid column chromatography.