Polyoma viral vector production cell lines and nucleic acids expressing dsRNA viral sequences

摘要

Polyoma viral vector production cell lines wherein said cells comprise a heterologous nucleic acid that expresses a double stranded RNA of at least 50 base pairs and which has significant homology to an essential sequence of a vertebrate virus. Also claimed are recombinant polyoma vectors carrying antisense sequences targeting viruses and methods of producing viral vectors, such as polyoma viral vectors e.g. SV40 viral vectors, host cells, compositions comprising viral vectors and therapeutic uses thereof, and vaccines against viruses such as HIV-1, hepatitis B or C (HBV or HCV).