Method for elucidating biological processes using FRET measurement methods

摘要

The method involves incorporating biological entities (X, Y), coupled with respective members of a pair of fluorescence resonance energy transfer (FRET) partners, in a measurement medium containing a lipid membrane such as a plasma membrane, where the members are located on both sides of the membrane and one member has a long lifespan. The membrane is excited at an excitation wavelength of an energy donor member, and a FRET signal or variations in the signal emitted in a culture medium is/are measured.