Wheat breeding methods using a diploid wheat genetic map made using the barley EST sequences

摘要

Is to provide a method for breeding wheat using diploid wheat genetic map that has been produced using a barley EST sequences. The breeding, including wheat step polymorphism detection for detecting a polymorphism of the DNA fragments amplified and amplification step, a primer set which is designed based on the barley EST sequences, amplifying a DNA as a template the genomic DNA of wheat is a method. Here, it is a DNA marker that is mapped on the genetic map to target the entire genome of the diploid wheat, the DNA fragments, using DNA markers more than 50 at least are mapped on the genetic map .