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Site-specific labeling of proteins for NMR studies
Site-specific labeling of proteins for NMR studies
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机译:用于NMR研究的蛋白质的位点特异性标记
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摘要
Methods of producing and/or analyzing spectroscopically labeled proteins, e.g., proteins site-specifically labeled with NMR active isotopes, spin-labels, chelators for paramagnetic metals, and the like, are provided. The labeled proteins are produced in translation systems including orthogonal aminoacyl tRNA synthetase/tRNA pairs. Methods for assigning NMR resonances, e.g., methods using isotopically labeled proteins, are also provided.
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