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Method Selecting Highly Specific Probes For HPV Genotype Analysis and the Probes Thereof
Method Selecting Highly Specific Probes For HPV Genotype Analysis and the Probes Thereof
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机译:用于HPV基因型分析的高特异性探针的选择方法及其探针
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摘要
A method for selecting a highly specific probe among a predetermined range of nucleotide sequences comprises:setting a group of nucleotide sequences among the predetermined range of nucleotide sequences; setting a range of nucleotide sequences in the group of nucleotide sequences; selecting first candidate probes having a certain length within the range of nucleotide sequences; selecting second candidate probes whose melting temperature with target nucleic acids for the first candidate probes is in an appropriate range; selecting third candidate probes whose melting temperature with a specific set of nucleotide sequences is lower than a hybridization temperature; and selecting fourth candidate probes, wherein a secondary structure of each fourth candidate probe has a melting temperature lower than the hybridization temperature by approximately 5° C. to approximately 10° C. and higher than a temperature that is lower than the hybridization temperature by approximately 5° C. to approximately 10° C.展开▼