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Primer set capable of specifically amplifying a target sequence found in 10 bacterial species and probe oligonucleotide specifically hybridizable with each target sequence of the 10 bacterial species
Primer set capable of specifically amplifying a target sequence found in 10 bacterial species and probe oligonucleotide specifically hybridizable with each target sequence of the 10 bacterial species
Provided are a primer set for amplifying a target sequence specific to Bordetella pertussis, Chlamydophila pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Legionella pneumophila, Moraxella catarrhalis, Mycoplasma pneumoniae, Pseudomonas aeruginosa, Stapylococcus aureus, and Streptococcus pneumoniae, the primer set including at least one oligonucleotide of 10 to 100 nucleotides in length, selected from the group consisting of oligonucleotides each of which comprises a fragment of at least 10 contiguous nucleotides present in a sequence as set forth in SEQ ID NO: 1 and at least one oligonucleotides of 10 to 100 nucleotides in length, selected from the group consisting of oligonucleotides each of which comprises a fragment of at least 10 contiguous nucleotides present in a sequence as set forth in SEQ ID NO: 2; and probes specific to a specific species of the 10 bacterial species.
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机译:提供了用于扩增百日咳博德特氏菌,肺炎衣原体,流感嗜血杆菌,肺炎克雷伯菌,肺炎军团菌,卡他莫拉菌,肺炎支原体,铜绿假单胞菌,铜绿假单胞菌和葡萄球菌特异的靶序列的引物组。肺炎链球菌,该引物组包括至少一个长度为10至100个核苷酸的寡核苷酸,选自由寡核苷酸组成的组,每个寡核苷酸均包含至少一个10个连续核苷酸的片段,该片段存在于序列中。 SEQ ID NO:1和至少一种长度为10至100个核苷酸的寡核苷酸,其选自由寡核苷酸组成的组,所述寡核苷酸各自包含存在于SEQ ID NO:2所示序列中的至少10个连续核苷酸的片段。 ;对10种细菌的特定物种具有特异性的探针。
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