首页>
外国专利>
Cell-free protein synthesis of deuterated proteins for structural and functional studies by NMR spectroscopy
Cell-free protein synthesis of deuterated proteins for structural and functional studies by NMR spectroscopy
展开▼
机译:氘化蛋白质的无细胞蛋白质合成,用于通过NMR光谱进行结构和功能研究
展开▼
页面导航
摘要
著录项
相似文献
摘要
A method for cell-free synthesis of at least partially deuterated proteins, peptides or assemblies thereof, in particular for NMR studies, is described. The method comprises at least the following steps: a) preparation of a cell extract under essentially non-deuterated conditions; b) exchanging D2O into the cell extract in a buffer exchange process to form a deuterated cell extract; c) cell-free synthesis using at least the deuterated cell extract, expressed and purified polymerase, at least partially deuterated amino acids and a plasimd or a linear template DNA encoding said protein or peptide; d) isolation and purification of the protein or peptide. Furthermore an extract for use in such a cell-free synthesis and a kit for use in such a cell-free protein synthesis is described
展开▼