首页> 外国专利> METHOD OF EVALUATING CELL FUNCTION, SYSTEM FOR EVALUATING CELL FUNCTION, FLUORESCENT MICROSCOPE SYSTEM, PHOTOTHERAPY METHOD AND PHOTOTHERAPY SYSTEM

METHOD OF EVALUATING CELL FUNCTION, SYSTEM FOR EVALUATING CELL FUNCTION, FLUORESCENT MICROSCOPE SYSTEM, PHOTOTHERAPY METHOD AND PHOTOTHERAPY SYSTEM

机译:评估细胞功能的方法,评估细胞功能的系统,荧光显微镜系统,光疗方法和光疗系统

摘要

A proposition of the present invention is to accurately evaluate a phototoxic property (cell function) concerning a living cell. To this end, an evaluating method of cell function of the present invention includes the operations of dyeing a specific site (41) of the living cell (40) with a fluorescent dye, irradiating the living cell (40) with light to measure changes in brightness of resulting fluorescence generated at an adjacent site (43) of the specific site (41), and evaluating the phototoxic property based on the brightness changes. In the event of functional depression in the specific site (41), the fluorescent dye cannot be retained therein and is extravasated into the adjacent site (43) through the membrane of the specific site. The present invention can measure the extent of this extravasation, making it possible to accurately evaluate the phototoxic property.
机译:本发明的目的是准确评估与活细胞有关的光毒性(细胞功能)。为此,本发明的细胞功能的评价方法包括以下操作:用荧光染料将活细胞(40)的特定部位(41)染色,用光照射活细胞(40)以测量其变化。在特定部位(41)的相邻部位(43)处产生的所得荧光的亮度,并基于该亮度变化评估光毒性。在特定位点(41)中功能降低的情况下,荧光染料不能保留在其中,而是通过特定位点的膜渗出到相邻位点(43)中。本发明可以测量这种外渗的程度,从而可以准确地评估光毒性。

著录项

  • 公开/公告号EP2003442A9

    专利类型

  • 公开/公告日2009-04-22

    原文格式PDF

  • 申请/专利权人 NIKON CORPORATION;SAKURAI TAKASHI;

    申请/专利号EP20070736970

  • 发明设计人 SAKURAI TAKASHI;IKI YOICHI;

    申请日2007-03-28

  • 分类号G01N21/64;

  • 国家 EP

  • 入库时间 2022-08-21 19:16:54

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