Antibody and hapten-protein conjugate utilized in an enzyme-linked immunosorbent assay (ELISA) for insecticide bistrifluron residues

摘要

A hapten-protein complex and an antibody used to an enzyme-linked immunosorbent analysis of an insecticide bistrifluron residue are provided to detect quickly and quantitively the insecticide bistrifluron using the antibody to a composite in which a specific hapten compound of bistrifluron and a protein are combined. A composite is manufactured by combining a hapten compound of an insecticide bistrifluron and a protein using an amino bond. The hapten compound of an insecticide bistrifluron includes a carboxyl group or an amino radical and a compound of haptens I, II, III, IV or V. The protein is a bovine serum albumin(BSA), a human serum albumin(HSA), a fibrinogen, an ovalbumin, a thyroglobulin or a keyhole limpet haemocyanin(KLH).