STABILIZATION METHODS FOR METALIC CONTAMINATED SOIL USING BY TRANSFORMATED ROBINIA PSEUDOACASIA VAR. UMBRACULIFERA

摘要

The present invention relates to a heavy-metal contaminated soil methods of purification for the mindung locust trees for using transgenosis, the mindung locust trees for especially preparing a transgenosis introduce heavy metal adsorption gene, using Agrobacterium, the mindung locust trees of transgenosis are enhanced using heavy-metal contaminated soil purification process involved in the mindung locust trees of transgenosis or the stable heavy-metal contaminated soil of heavy-metal contaminated soil cleaned by planting heavy metal tolerance ability. ;The heavy-metal contaminated soil method of purification that the mindung locust trees of transgenosis are used according to the present invention is 1 to 10 wt% sucrose, 0.1~0.5mg/L BA (6-benzyladenine), 0.1~0.5mg/L TDZ (Thidiazuron) are the following steps are included: the regeneration for the locust tree mindung being added to is MS media; Heavy metal-resistant genes, YCF, YHL introduce ZntA in Agrobacterium and are inserted into after mindung locust trees; By plant transgene mindung locust tree heavy metal pollutions place can adsorb heavy metals in soil and it is characterized in that heavy-metal contaminated soil purification and stabilization.