QUANTIFICATION EGFP FLUORESCENCE AS AN INDICATOR OF ARSENITE DEVELOPMENTAL TOXICITY IN MOSAIC HEAT SHOCK PROTEIN 70 TRANSGENIC ZEBRAFISH

摘要

A method for quantitatively analyzing the absolute value of fluorescence intensity and the amount of EGFP (Enhanced Green Fluorescent Protein) in a transgenic fish is provided to quantitatively measure the harmfulness of toxic material. A method for qualitatively analyzing the amount of GFP or EGFP of transgenic fish comprises: a first step of measuring the fluorescence intensity based on the amount of the GFP and purified GFP and forming standard linear equation; a second step of inserting a toxic material reactive gene promoter in a vector which expresses GFP or EGFP to produce the transgenic fish; a third step of measuring the fluorescence intensity which the transgenic fish expresses; a fourth step of calculating the amount of GFP or EGFP corresponding to the fluorescence intensity; and a step of measuring the amount of purified GFP or EGFP and forming standard linear equation.