SIVmac239 Immunogenic Plasmids And AIDS DNA Vaccine Containing The Same

摘要

PURPOSE: Immunogenicity plasmids of SIVmac 239 and AIDS DNA vaccines containing the same are provided, thereby effectively preventing and treating AIDS using the DNA vaccine containing the immunogenicity plasmid and adjuvant plasmid. CONSTITUTION: A basis vector pGX10 for producing the immunogenicity plasmid, has the nucleotide sequence of SEQ ID NO: 60. An immunogenicity plasmid pGX10-SIV/GE contains genes gag, dpol and env derived from virus SIVmac 239 and adjuvant gene rev. An immunogenicity plasmid pGX10-SIV/dpol, wherein a gene pol derived from SIVmac 239, encoding reverse transcriptase(RT) and integrase(INT) and a signal sequence of protein to be secreted, fused with the 3'-terminal of the gene pol, are operably linked to the vector pGX10, wherein the gene pol is mutated to inhibit the activity of integrase; the mutated region of the gene pol is nucleotides 5130 to 5132 and nucleotides 5133 to 5135 substituted with a codon encoding serine; the signal sequence of protein to be secreted is signal sequence of glycoprotein; and the signal sequence of glycoprotein is derived from Herpes simplex virus(HSV).