De novo formation of artificial chromosomes of 3 - fabricated mult strength constructs in primary cells for the production of organs for transplantation medicine / xenotransplantation

摘要

The present invention relates to a process for the introduction of multiple transgenic into a primary cell and to a process for the preparation of multi - of transgenic of primary cells, tissues and / or animals by the use of a nucleic acid construct which is able to form de novo artificial chromosomes. The present invention further relates to the use of the multi - transgenic primary cells, tissues and / or animals, preferably in the connection with the xenotransplantation. The nucleic acid constructs, which are capable of de novo artificial chromosomes to form, are used as efficient means for the transfer of multiple human transgenic and, therefore, lead to generation of animals than in the organ donor xenotransplanation.