JUVENILE HORMONE ACID METHYLTRANSFERASE GENE AND UTILIZATION THEREOF

摘要

PPROBLEM TO BE SOLVED: To provide a highly safe insect growth regulator specifically acting to an insect. PSOLUTION: A Juvenile hormone acid methyltransferase gene is cloned from a cDNA originated from a corpus allatum of Bombyx mori by a differential display method. It is found that a recombinant protein expressed by the colon bacillus transformed by a vector DNA incorporated with the gene has Juvenile hormone acid methyltransferase activities. The Juvenile hormone acid methyltransferase genes originated from Drosophila melanogaster, Anopheles gambiae, Spodoptera litura and Helicoverpa armigera are found based on the homology in the amino acid sequences. Also, it is found that the proteins encoded by the Juvenile hormone acid methyltransferase genes originated from the Drosophila melanogaster, the Anopheles gambiae, the Spodoptera litura and the Helicoverpa armigera have the Juvenile hormone acid methyltransferase activities. PCOPYRIGHT: (C)2010,JPO&INPIT