Chromatographic separation methods of nucleic acid, as well as high-affinity ion exchange matrix and novel peptide

摘要

(57) Abstract The hydroxyl basis or primary, second class or there is a third class amino group 2 or 3 atoms in the carbon atom which leaves from the amino nitrogen inside the anion exchange body (i) b. ligand which is the ligand which a.(i) primary, second class or includes the third class amino group, the (ii) organic polymer (matrix) and it shares connects, toransuhuerin, ovalbumin 1, ovalbumin 2, - lactoglobulin 1, inside - lactoglobulin 2, at least in one protein and in c. anion exchange body (i) maximum liquation ionic strength of the pH2-14 range, from the ionic strength which the fourth class relative exchanger needsThe method of separating bepuchido or nuclear acid due to anion exchange body (i) the fact that it becomes high is feature. kuromatohuokashingu or the ECTEOLA cellulose it is not included. By structural(i) [in formula, R as for_1-6, the new anion exchange body ether oxygen atom, it is the hydrocarbon radical which includes hydroxyl basis and amino group of type which differs,]. X is OH or the alkoxy or amino. B R_{1-6 the} basis or each R_1-6 hydrogen is the ligand arm which is substituted. R₅ and R_{6 the} nitrogen atom is included in one, if is, hydroxy or alkoxy group is included to one basis. The ECTEOLA cellulose or the deformation type where both of R₅ and R₆ are selected from methyl and ethyl are not included