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Cyanine-Based ProbeTag-Peptide Pair Fluorescence Protein Imaging and Fluorescence Protein Imaging Methods
Cyanine-Based ProbeTag-Peptide Pair Fluorescence Protein Imaging and Fluorescence Protein Imaging Methods
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机译:基于花青的探针标记肽对荧光蛋白成像和荧光蛋白成像方法
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摘要
A molecular probe comprises two arsenic atoms and at least one cyanine based moiety. A method of producing a molecular probe includes providing a molecule having a first formula, treating the molecule with HgOAc, and subsequently transmetallizing with AsCl3. The As is liganded to ethanedithiol to produce a probe having a second formula. A method of labeling a peptide includes providing a peptide comprising a tag sequence and contacting the peptide with a biarsenical molecular probe. A complex is formed comprising the tag sequence and the molecular probe. A method of studying a peptide includes providing a mixture containing a peptide comprising a peptide tag sequence, adding a biarsenical probe to the mixture, and monitoring the fluorescence of the mixture.
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