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Application of RNA Interference Targeting dhfr Gene, to Cell for Producing Secretory Protein
Application of RNA Interference Targeting dhfr Gene, to Cell for Producing Secretory Protein
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机译:RNA干扰靶向dhfr基因在分泌蛋白生产细胞中的应用
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摘要
Biological materials are applied to a CHO cell or the like for enhancing production of a species of protein. The biological materials includes an expression vector and a silencing vector, the expression vector including a dhfr gene of a species of mammal and a gene encoding the species of protein, the silencing vector including a DNA fragment for inducing a RNA interference in the CHO cell to reduce expressions of both exogenous dhfr gene and endogenous dhfr gene after the biological material is applied to the CHO cell, and the CHO cell is thus not limited to dhfr gene deficient type. The DNA fragment consists of nucleotides characterizing a segment of a dhfr gene of the CHO cell and a segment of a dhfr gene of the species of mammal.
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