'PRIMERS FOR DEVELOPING GENOTYPE SPECIFIC MARKER IN PIPER NIGRUM'

摘要

This invention relates to a primers for developing genotype specificmarker in Piper nigrum a) 5'-ACCTCCTCTTTGTCACTCACTTC-3',Pngfi5'-CAGCCTTCCGGACTTCACCAC-S'Pngr1,b)5'-GCAGCGACAGCCAGCACA-3'Pncf2,5'-CCATCGTACCATTCAATCATC-3 Pncn, Wherein the designing based on the flanking sequence of the micro satellite region using primer premier, Tsp 5091 adapters are used to bind the restriction sites created by the Tsp enzyme in DNA fragments, the said oligos are labeled with bio tin and the beads are coated in streptavidin which binds with the biotin protein and a magnet is used to separate the streptavidin bound oligo DNA from unbound.