METHOD FOR THE PRODUCTION OF RECOMBINANT ANTIGENS FOR USE AS VACCINES AGAINST CYSTICERCOSIS-TAENIASIS.

摘要

The present invention consists in using the recombinant protein expression system in embryogenic cells of papaya for the expression and production of protective recombinant proteins against cysticercosis and taeniasis. Three genetic constructions were performed, wherein the sequences encoding for peptides KETc1, KETc7 and KETc12 from the cysticercus T. crassiceps were independently donated to the expression vector pUl235-5.1. Said constructions were used for transforming, by biobalistics, embryogenic papaya (Carica papaya) callus. The embryogenic callus were co-transformed with each one of the genetic construction in the vector pUl235-5.1 and the vector pWRG1515 which contain the GUS reporter gene that encodes for the (-glucuronidase) enzyme (Christou et al., 1995). The transformed callus were selected and isolated in a selective medium with antibiotics. The ability of expression and immunogenicity of the produced clones were confirmed. Based on the immunogenic ability of the clones, the clones 6p (KETC12.6 His), 9p (KETc1. 6 His) and 23p (KETc7) were selected therefrom, the same being spread, preserved and started the regeneration process in vitro.