MONOLITHIC MATRIX FOR SEPARATING NUCLEIC ACIDS BY REVERSE-PHASE ION-PAIR HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

摘要

The present inventionprovides monolithicpolymer matrices for theseparation of bio-organicmolecules by liquidchromatography. Inone embodiment, thematrix is formed from apolymerization mixtureincluding (i) a hydrophobicmonomer, (ii) a crosslinkingagent, and (iii) a porogenicsolvent or mixture ofporogenic components.The monolithic matricesof the invention areparticularly useful forresolving polynucleotides(e.g., DNA and/or RNA)in samples by way ofreversed-phase ion-pairingchromatography.