PROBES FOR IMAGING PROTEIN PHOSPHORYLATION AND DEPHOSPHORYLATION AND METHOD FOR DETECTING AND DETERMINING PROTEIN PHOSPHORYLATION AND DEPHOSPHORYLATION USING THE SAME

摘要

A widely applicable method of detecting and quantifying thephosphorylation/dephosphorylation of a protein in cells whereby thephosphorylation/dephosphorylation of a protein in vital cells, animals,plants, etc. can be non-destructively monitored and spatial and time-courseanalyses can be performed. In this method, use is made of a probe forvisualizing phosphorylation/dephosphorylation characterized in that a tandemfusion unit, which has a substrate domain having aphosphorylation/dephosphorylation site and a phosphorylation-recognizingdomain attached thereto via a linker sequence, is located between a donorchromophore and an acceptor chromophore in fluorescence resonance energytransfer.