EXPRESSION VECTOR OF EUKARYOTIC CELLS OR PROKARYOTIC CELLS-DERIVED MEMBRANE PROTEIN AND METHOD FOR PREPARING THE MEMBRANE PROTEIN USING THE SAME

摘要

PURPOSE: A membrane protein expression vector having major envelop protein P9 of cystovirus phi6 is provided to effectively overexpress membrane protein and use for bioconversion.;CONSTITUTION: A membrane protein expression vector contains major envelope protein P9 gene of cystovirus phi6, multi-cloning site(MCS) for inserting a target membrane protein and protease recognistion site between the P9 gene and MCS. The P9 protein has amino acid of sequence number 1 or 2. A gene of P9 protein has a base sequence of sequence number 3 or 4. The expression vector comprises promoter from 5' to 3', gene of P9 protein, protease recognition site, MCS and histidine tag in a translational fusion form. The expression vector is pRphi6-9. A transformed cell using the expression vector is E.coli EPI300/pRphi6-9(deposit number: KCTC 11373BP).;COPYRIGHT KIPO 2010