METHOD FOR SCREENING DNA METHYLTRANSFERASE INHIBITOR USING DROSOPHILA LUCIFERASE GENE AS A REPORTER GENE

摘要

PURPOSE: A vector comprising VHL(von Hippel-Lindau) promoter and Drosophila luciferase gene as a reporter gene and a method for screening DNA methyltansferase inhibitor using the same are provided to screen a therapeutic agent for neural disorder, blood disease, or tumor.;CONSTITUTION: A recombinant expression vector comprises a VHL(von Hippel-Lindau) promoter of sequence number 1, 2, 3, 4, or 5, and fluorescence protein gene which is fused to the promoter. The fluorescence protein is Drosophila luciferase, enhsnce green fluorescent protein(EGFP), or cyan fluorescent protein(CFP). The VHL promoter is a promoter of sequence number 5. The fluorescence protein is Drosophila luciferase. A transformed cell line is obtained by transforming a pRL-SV40 plasmid vector containing the recombinant expression vector and renila-luciferase. A method for screening a DNA methyltransferase inhibitor comprises: a step of culturing transformed cell line in a plate; a step of adding a sample to detect and culturing in hypoxia condition; and a step of measuring fluorescence and renila-luciferase fluorescence.;COPYRIGHT KIPO 2010