RECOMBINANT flagellin, plasmid DNA, recombinant strains of flagella archaea, METHOD FOR PRODUCING nanostructured material, COMPOSITION AND KIT FOR ITS basis for the formation nanostructured coatings (optional)

摘要

FIELD: chemistry.;SUBSTANCE: invention relates to the field of biotechnology and can be used for obtaining a nanostructured material based on recombinant flagella of archea H. salinarum, bound with metal ions or nanoparticles. Transformed with a recombinant plasmid cells of archea are grown, flagella, containing peptide inserts for binding with metal ions or nanoparticles, are separated. The surface of flagella is modified by binding peptide inserts with the said ions or nanoparticles with further washing, drying and packaging of the obtained material. The plasmid construction contains recombinant genes for synthesis of A1 and A2 flagella-forming flagellins. A sequence of flagellin A1 and/or flagellin A2 contains a peptide insert for selective binding metal ions or nanoparticles, where the location of the peptide insert is determined in the region between the first and second sites of glycosylation, located in flagellin A1 between position 86 and position 96 SEQ ID NO: 2, and in flagllin A2 between position 82 and position 92 SEQ ID NO: 3.;EFFECT: invention makes it possible to obtain the nanostructured material with improved adhesive properties, resistant with respect to impact of high temperatures.;10 cl, 7 dwg, 7 ex