RECOMBINANT DNA, WHICH CODES FUNCTIONALLY ACTIVE HYBRID PROTEIN GL7ACA-ACYLASE WITH CHITIN-BINDING DOMAIN (BrdGL7ACA-cbd) AND RECOMBINANT PLASMID pSVH0108, PROVIDING FOR ITS SYNTHESIS IN CELLS Escherichia coli, RECOMBINANT STRAIN Escherichia coli BL21(DE3)/pSVH0108-PRODUCER BrdGL7ACA-cbd

摘要

FIELD: biotechnologies.;SUBSTANCE: recombinant DNA is produced, which codes functionally active hybrid protein (BrdGl7ACA-cbd), consisting of amino-acid sequence of acylase glutaryl-7- aminocephalosporanic acid of strain Brevundimonas diminuta All-Russian collection of industrial microorganisms B-1297 and chitin-binding domain of chitinase Al Bacillus circulans. Recombinant plasmid pSVH0108 is constructed for expression of BrdGl7ACA-cbd in cells E.coli, containing sequence of recombinant DNA that codes hybrid protein under control of promotor and terminator of RNA-polymerase of phage T7. As a result of E.coli strain tranformation with this recombinant plasmid and selection of transformed clones, a new strain E.coli BL21(DE3)/pSVH0108 cbd is produced - producer of hybrid protein BrdGl7ACA-cbd.;EFFECT: high yield of recombinant ferment.;4 dwg, 2 tbl, 7 ex