SYPHILIS DIAGNOSTIC TECHNIQUE BY SIMULTANEOUS DETECTION OF REAGINIC AND TREPONEMA-SPECIFIC T PALLIDUM ANTIBODIES ON MICROSCOPE ALDEHYDE SLIDES

摘要

FIELD: medicine.;SUBSTANCE: invention refers to medicine, particularly to infectious diseases. It involves simultaneous analysis of blood serum for reaginic and treponema-specific antibodies to cardiolipin antigen and recombinant T pallidum protein complex with molecular weight 15, 17, 39, 41, 42, 44.5, 47 kDa, immobilised on microscope aldehyde slides to detect antibodies by a conjugate solution containing Cy5 phosphor tagged human IgG antibodies and Cy3 phosphor tagged human IgM antibodies; slide scanning in a multichannel biochip scanner; automatic data analysis in a program used to convert fluorescent signals to digital positiveness coefficients; and qualitative presentation. Reaginic and treponema-specific antibodies found in a sample indicate syphilis, while no reaginic and treponema-specific antibodies found in the sample shows the absence of syphilis in a patient.;EFFECT: method allows for differentiated detection of antibodies to the most immunogenic T Pallidum antigens produced at the different stages of disease, for the objective automated data record, for cutting examination and diagnosing time.;4 cl, 2 ex