SSR MARKER PRIMER PAIR FOR IDENTIFYING SPECIES OF TIMOTHY AND METHOD FOR IDENTIFYING SPECIES OF TIMOTHY USING PRIMER SET

摘要

PROBLEM TO BE SOLVED: To provide a primer pair for identifying species of timothy simply, quickly and accurately, a specific marker gene and a method for identifying the species.SOLUTION: The primer pair is designed by using base sequences at both sides of an SSR region on a genome of timothy. The specific marker gene is obtained by bulking the timothy to be an object using the primer pair to eliminate a variation of genes between individuals and specifically amplifying a species-specific SSR by PCR. The method for identifying species includes: detecting a difference in length of amplified marker gene fragments to identify species. The method for identifying species of the timothy includes a fewer number of necessary processes in comparison with a species differentiation method of conventional morphological characteristics, heading date, etc., and uses bulk DNAs of a plurality of individuals as a template DNA.