The plow motor which is induced by the rearrangement vector for chromosome specification region removal and the removal mannered null arabinose of the chromosome

摘要

Disclosed herein are a recombinant vector for deletion of specific chromosomal regions and a method for deletion of targeted microbial chromosomal regions using the same. Specifically, the recombinant vector comprises an arabinose-inducible promoter; a gene encoding a protein involved in lambda (»)-red recombination; a rhamnose-inducible promoter; and a gene encoding the I- Sce I endonuclease. The present invention enables a convenient, rapid and markerless successive deletion of specific genes of microbes, as compared to a conventional method.