'A FLOW THROUGH ENZYME IMMUNOASSAY KIT FOR THE SCREENING OF HIV 1, HIV 2 ANTIBODIES AND FOR EARLY DETECTION OF HIV 1 P24 ANTIGEN'

摘要

The present invention relates to a 4th generation diagnostic kit for an early detection of HIV1 p24 antigen, HIV-1 antibodies and HIV-2 antibodies using flow through enzyme immunoassay kit so that a more accurate result will be obtained. The device used is a flow through assay device which comprises of four dots in which recombinant antigens or synthetic peptides or biotinylated recombinant antigens or biotinylated synthetic peptides, native antigens of gp41, gp 120, gp160, gp36 and anti HIV 1 p24 antibody are immobilized. The fourth dot is control dot which is immobilized with anti species IgG. The conjugate used is HIV 1 and HIV 2 antigen and HIV 1 p24 antibodies conjugated with Horse radish peroxidase.