Mass labels for biomolecules containing a 2,6-dimethyl-piperidin-1-yl methylene or a pyrimidin-2-yl thiomethylene mass marker moiety and a succinimid-oxy-carbonyl reactive functional group

摘要

Disclosed is a set of two or more mass labels, wherein each label in the set is a mass label for labelling and detecting a biological molecule by mass spectroscopy, which label comprise the following structure: X-L-M wherein X is a mass marker moiety of formula (X) wherein the cyclic unit is aromatic or aliphatic and comprises from 0-3 double bonds independently between any two adjacent atoms; each Z is independently N, N(R1), C(R1), CO, C-O(R1), C(R1)2, O or S; X is N, C or C(R1) each R1 is independently H, a substituted or unsubstituted straight or branched Cr C6 alkyl group, a substituted or unsubstituted aliphatic cyclic group, a substituted or unsubstituted aromatic group or a substituted or unsubstituted heterocyclic group; and y is an integer from 0-10, L is a cleavable linker comprising an amide bond and M is a mass normalization moiety; and wherein each mass normalisation moiety ensures that a mass label has a desired aggregate mass.